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primary antibodies directed against neun  (Millipore)


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    Structured Review

    Millipore primary antibodies directed against neun
    Primary Antibodies Directed Against Neun, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+against+neun/anti+neun/pm40499883-73-10-21
    Average 90 stars, based on 1 article reviews
    primary antibodies directed against neun - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Salvianolic acid B ameliorates neuroinflammation and neuronal injury via blocking NLRP3 inflammasome and promoting SIRT1 in experimental subarachnoid hemorrhage.
    Article Snippet: In brief, the sections were Frontiers in Immunology 03 permeabilized with 0.5% Triton X-100 (9002-93-1, Thermo Scientific) and then blocked with 10% BSA. .. Then the tissue sections were incubated with the primary antibody against NeuN (MAB377, Millipore), and then incubated with TUNEL reaction mixture. ..

    Article Title: MicroRNA-335-5p suppresses voltage-gated sodium channel expression and may be a target for seizure control
    Article Snippet: AAV9-injected mice were perfused with PBS, brains post-fixed in 4% paraformaldehyde, sectioned (12 μm coronal) on a cryostat (CM1900, Leica), and mounted on glass slides. .. Sections were blocked in 1% BSA for 1.5 h followed by incubation overnight with the primary antibody against NeuN (Millipore). .. On the next day, slices were washed and then incubated with fluorescent secondary antibody (Invitrogen) for 2 h. Then, sections were rinsed again, stained with DAPI, and mounted and imaged using a Leica DM4000 epifluorescence microscope.

    Article Title: Asialoerythropoetin is not effective in the R6/2 line of Huntington's disease mice
    Article Snippet: .. The sections were preincubated for 1 h in 5% normal horse serum (NHS) and 0.25% Triton X-100 in PBS and then incubated overnight at room temperature with a primary antibody against NeuN (diluted 1:1000, Chemicon, Temecula, CA, USA) in PBS containing 2% NHS. .. After incubation with a biotinylated horse anti-mouse antibody (1: 200; Dakopatts, Copenhagen, Denmark) for 1 h, the bound antibodies were visualized using an avidin-biotin-peroxidase complex system (Vectastain ABC Elite Kit, Vector labs, Burlingame, CA, USA).

    Article Title: MicroRNA-335-5p suppresses voltage-gated sodium channel expression and may be a target for seizure control.
    Article Snippet: AAV9- injected mice were perfused with PBS, brains post- fixed in 4% paraformaldehyde, sectioned (12 μm coronal) on a cryostat (CM1900, Leica), and mounted on glass slides. .. Sections were blocked in 1% BSA for 1.5 h followed by incubation overnight with the primary antibody against NeuN (Millipore). .. On the next day, slices were washed and then incubated with fluorescent secondary antibody (Invitrogen) for 2 h. Then, sections were rinsed again, stained with DAPI, and mounted and imaged using a Leica DM4000 epifluorescence microscope.

    TUNEL Assay:

    Article Title: Salvianolic acid B ameliorates neuroinflammation and neuronal injury via blocking NLRP3 inflammasome and promoting SIRT1 in experimental subarachnoid hemorrhage.
    Article Snippet: In brief, the sections were Frontiers in Immunology 03 permeabilized with 0.5% Triton X-100 (9002-93-1, Thermo Scientific) and then blocked with 10% BSA. .. Then the tissue sections were incubated with the primary antibody against NeuN (MAB377, Millipore), and then incubated with TUNEL reaction mixture. ..

    other:

    Article Title: Functional roles of neuromedin B and gastrin-releasing peptide in regulating itch and pain in the spinal cord of non-human primates
    Article Snippet: The tissue sections were washed in PBS containing 0.3% Triton X-100 (Sigma-Aldrich) and treated with TrueBlack Lipofusin Autofluorescence Quencher (Biotium, Fremont, CA, USA) for 2 min. After washing with PBS, we blocked the tissues with PBS containing 3% normal donkey serum (Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at room temperature for 1 h. The sections were incubated with a primary antibody against GRPR (rabbit polyclonal, 1:500 dilution; MC-831, MBL International, Woburn, MA, USA), NMBR (rabbit polyclonal, 1:500 dilution; ABR-004, Alomone Labs, Jerusalem, Israel), and NeuN (mouse monoclonal, 1:500 dilution; MAB377, MilliporeSigma, Burlington, MA, USA; markers for neuronal nuclei) at 4 °C overnight.

    Article Title: PHGDH-mediated serine synthesis in astrocytes supports neuroinflammation by sustaining NADH level to promote histone acetylation
    Article Snippet: Brain tissues were fixed in 4% PFA in phosphate buffer for 12 h at room temperature (RT) and incubated at 4 °C in phosphate buffer containing 30% sucrose for 48 h. The cryosections were permeabilized and blocked in PBS containing 0.3% triton and 10% BSA for 2 h and incubated at 4 °C overnight with the primary antibody against PHGDH (1:1000), GFAP (1:2000, DAKO), Iba1(1:1000, Wako), NeuN (1:1000, Millipore), P-P65(1:1000, CST 3033).

    Tissue Homogenization:

    Article Title: Integrated multimodal cell atlas of Alzheimer’s disease
    Article Snippet: .. Briefly, after tissue homogenization, isolated nuclei were stained with a primary antibody against NeuN (FCMAB317PE, Millipore-Sigma) to label neuronal nuclei. .. Nuclei samples were analyzed using a BD FACS Aria flow cytometer and nuclei were sorted using a standard gating strategy to exclude multiplets .

    Isolation:

    Article Title: Integrated multimodal cell atlas of Alzheimer’s disease
    Article Snippet: .. Briefly, after tissue homogenization, isolated nuclei were stained with a primary antibody against NeuN (FCMAB317PE, Millipore-Sigma) to label neuronal nuclei. .. Nuclei samples were analyzed using a BD FACS Aria flow cytometer and nuclei were sorted using a standard gating strategy to exclude multiplets .

    Staining:

    Article Title: Integrated multimodal cell atlas of Alzheimer’s disease
    Article Snippet: .. Briefly, after tissue homogenization, isolated nuclei were stained with a primary antibody against NeuN (FCMAB317PE, Millipore-Sigma) to label neuronal nuclei. .. Nuclei samples were analyzed using a BD FACS Aria flow cytometer and nuclei were sorted using a standard gating strategy to exclude multiplets .



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    97
    Synaptic Systems primary antibodies against neun
    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
    Primary Antibodies Against Neun, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Servicebio Inc primary antibody 178 against neun
    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
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    Millipore primary antibodies directed against neun
    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and <t>NeuN</t> (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in <t>(b)</t> <t>GFAP-positive</t> astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.
    Primary Antibodies Directed Against Neun, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore primary antibody against neun
    A , B The immunofluorescence staining of <t>PHGDH,</t> <t>GFAP,</t> Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.
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    Millipore primary antibody against the neuron-specific protein neun mab-377
    A , B The immunofluorescence staining of <t>PHGDH,</t> <t>GFAP,</t> Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.
    Primary Antibody Against The Neuron Specific Protein Neun Mab 377, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Synaptic Systems primary antibodies against neun for neurons
    A , B The immunofluorescence staining of <t>PHGDH,</t> <t>GFAP,</t> Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.
    Primary Antibodies Against Neun For Neurons, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore primary antibodies against neun
    A , B The immunofluorescence staining of <t>PHGDH,</t> <t>GFAP,</t> Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.
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    Cell Signaling Technology Inc primary antibodies against neuronal nuclear antigen
    A , B The immunofluorescence staining of <t>PHGDH,</t> <t>GFAP,</t> Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.
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    A , B The immunofluorescence staining of <t>PHGDH,</t> <t>GFAP,</t> Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.
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    Image Search Results


    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and NeuN (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.

    Journal: bioRxiv

    Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

    doi: 10.64898/2026.01.22.698893

    Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CeA of AIE rats. Ribosomal RNA (rRNA, magenta) was used as a cytoplasmic label for single-cell segmentation, and NeuN (cyan) was used to identify neuronal cells. White arrows indicate NeuN-positive cells. Right: Images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the CeA of AIE rats. All the images in (a-c) are maximum intensity projection (MIP) images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d ) neurons ( p = 2.78 x 10 -5 ), (e) astrocytes ( p = 0.790), and (f) microglia ( p = 0.371) in the CeA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± standard error of the mean (SEM) with individual data points shown as black diamonds.

    Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

    Techniques:

    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the BLA of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the BLA of AIE rats. All the images in (a-c) are MIP images (∼8.6 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.990), (e) astrocytes ( p = 0.371), and (f) microglia ( p = 0.792) in the BLA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Journal: bioRxiv

    Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

    doi: 10.64898/2026.01.22.698893

    Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the BLA of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) and (c) IBA1-positive microglia (white arrows) in the BLA of AIE rats. All the images in (a-c) are MIP images (∼8.6 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.990), (e) astrocytes ( p = 0.371), and (f) microglia ( p = 0.792) in the BLA of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

    Techniques: Labeling

    (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CA1 of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels, with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) in the CA1 and (c) IBA1-positive microglia (white arrows) in the DG of AIE rats. All the images in (a-c) are MIP images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.009), (e) astrocytes ( p = 0.174), and (f) microglia ( p = 0.421) in the hippocampus of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). Individual data points are the average of 9 cells, 3 each from the CA1, CA3, and DG. In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Journal: bioRxiv

    Article Title: Super-resolved, three-dimensional spatial transcriptomics reveals cell-type and brain-region-specific modulation of key epitranscriptomic switches following adolescent alcohol exposure

    doi: 10.64898/2026.01.22.698893

    Figure Lengend Snippet: (a) Left: RESOLVE-FISH images showing Mettl3 mRNA (green) in the CA1 of AIE rats. The cytoplasm is labeled by rRNA (magenta), and all neuronal cells (white arrows) are marked by NeuN (cyan). Right: images corresponding to the NeuN, rRNA, Mettl3 mRNA, and DAPI channels, with dashed lines outlining the cell bodies. (b, c) RESOLVE-FISH images showing Mettl3 mRNA (green) in (b) GFAP-positive astrocytes (white arrows) in the CA1 and (c) IBA1-positive microglia (white arrows) in the DG of AIE rats. All the images in (a-c) are MIP images (∼6.9 µm in z-range, pre-expansion scale). Scale bars, 10 µm (35 µm). (d-f) Cytoplasmic Mettl3 mRNA puncta per cell across (d) neurons ( p = 0.009), (e) astrocytes ( p = 0.174), and (f) microglia ( p = 0.421) in the hippocampus of AIS and AIE rats (n = 6 per group, male and female, two-sample t-test). Individual data points are the average of 9 cells, 3 each from the CA1, CA3, and DG. In all plots, data are presented as mean ± SEM with individual data points shown as black diamonds.

    Article Snippet: Primary antibodies against NeuN [guinea pig, 266004, Synaptic Systems (SYSY)], GFAP (guinea pig, 173308, SYSY), or IBA1 (chicken, 234009, SYSY) were used to label cells of each cell type in the sample ( Supplemental Table S1 ).

    Techniques: Labeling

    A , B The immunofluorescence staining of PHGDH, GFAP, Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.

    Journal: Cell Death & Disease

    Article Title: PHGDH-mediated serine synthesis in astrocytes supports neuroinflammation by sustaining NADH level to promote histone acetylation

    doi: 10.1038/s41419-025-07732-8

    Figure Lengend Snippet: A , B The immunofluorescence staining of PHGDH, GFAP, Iba-1, NeuN and DAPI in brain tissue of C57BL/6 mice before and after LPS stimulation and the statistics of colocalization calculated as Pearson’s correlation coefficient, r. Scale, 100 μm, n = 7. C immunofluorescence staining representation of PHGDH, GFAP and Iba-1 in substantia nigra of brain tissue of C57BL/6 mice induced by MPTP. Scale, 100 μm, n = 4. D Statistics of relative PHGDH fluorescence intensity per astrocyte. 54 cells from 6 mice. Statistics of relative PHGDH fluorescence intensity per microglia. 45 cells from 4 mice. The data are means ± SD, for all panels: * P < 0.05, ** P < 0.01, *** P < 0.001, n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ) and one-way ANOVA analysis followed by Student’s t -test ( D ). All data are representative of or combined from at least three independent experiments.

    Article Snippet: Brain tissues were fixed in 4% PFA in phosphate buffer for 12 h at room temperature (RT) and incubated at 4 °C in phosphate buffer containing 30% sucrose for 48 h. The cryosections were permeabilized and blocked in PBS containing 0.3% triton and 10% BSA for 2 h and incubated at 4 °C overnight with the primary antibody against PHGDH (1:1000), GFAP (1:2000, DAKO), Iba1(1:1000, Wako), NeuN (1:1000, Millipore), P-P65(1:1000, CST 3033).

    Techniques: Immunofluorescence, Staining, Fluorescence

    A Representative images of PHGDH, GFAP and DAPI immunofluorescence staining in primary astrocytes treated with LPS or PBS. Scale bar, 20 μm, n = 3. B Representative immunoblotting of phosphorylation (p-) or total protein of astrocyte lysates treated with DMSO or NCT-503 in the presence or absence of LPS, n = 3. C Representative images of GFAP, P-P65 and DAPI immunofluorescence staining after astrocytes treated with DMSO or NCT-503 and treated PBS or LPS for 1 h. Scale bar, 10 μm, n = 3. The data are means ± SD, for all panels: n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ).

    Journal: Cell Death & Disease

    Article Title: PHGDH-mediated serine synthesis in astrocytes supports neuroinflammation by sustaining NADH level to promote histone acetylation

    doi: 10.1038/s41419-025-07732-8

    Figure Lengend Snippet: A Representative images of PHGDH, GFAP and DAPI immunofluorescence staining in primary astrocytes treated with LPS or PBS. Scale bar, 20 μm, n = 3. B Representative immunoblotting of phosphorylation (p-) or total protein of astrocyte lysates treated with DMSO or NCT-503 in the presence or absence of LPS, n = 3. C Representative images of GFAP, P-P65 and DAPI immunofluorescence staining after astrocytes treated with DMSO or NCT-503 and treated PBS or LPS for 1 h. Scale bar, 10 μm, n = 3. The data are means ± SD, for all panels: n.s. no significance by two-way ANOVA analysis followed by Bonferroni Test ( B ).

    Article Snippet: Brain tissues were fixed in 4% PFA in phosphate buffer for 12 h at room temperature (RT) and incubated at 4 °C in phosphate buffer containing 30% sucrose for 48 h. The cryosections were permeabilized and blocked in PBS containing 0.3% triton and 10% BSA for 2 h and incubated at 4 °C overnight with the primary antibody against PHGDH (1:1000), GFAP (1:2000, DAKO), Iba1(1:1000, Wako), NeuN (1:1000, Millipore), P-P65(1:1000, CST 3033).

    Techniques: Immunofluorescence, Staining, Western Blot, Phospho-proteomics